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The disulfidptosis induced by M@Cys-Gox-Mn NPs. a) Representative bright-field microscopic images of T24 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. NPs: M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). <t>Z-VAD-fmk:</t> Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. b) Averaged T24 cell numbers in different groups in a). c) Representative bright-field microscopic images of 5637 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). Z-VAD-fmk: Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. d) Averaged 5637 cell numbers in different groups in a). ∗∗∗∗ p < 0.0001.
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The disulfidptosis induced by M@Cys-Gox-Mn NPs. a) Representative bright-field microscopic images of T24 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. NPs: M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). Z-VAD-fmk: Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. b) Averaged T24 cell numbers in different groups in a). c) Representative bright-field microscopic images of 5637 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). Z-VAD-fmk: Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. d) Averaged 5637 cell numbers in different groups in a). ∗∗∗∗ p < 0.0001.

Journal: Materials Today Bio

Article Title: SLC7A11-independent disulfidptosis induced by a two-pronged delivery strategy for bladder cancer chemotherapy and cisplatin-resistance reverse

doi: 10.1016/j.mtbio.2025.102547

Figure Lengend Snippet: The disulfidptosis induced by M@Cys-Gox-Mn NPs. a) Representative bright-field microscopic images of T24 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. NPs: M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). Z-VAD-fmk: Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. b) Averaged T24 cell numbers in different groups in a). c) Representative bright-field microscopic images of 5637 cells after incubating with M@Cys-Gox-Mn NPs and/or different inhibitors for 7 h. M@Cys-Gox-Mn NPs (Gox-equivalent dose: 0.2 μg/ml). β-mercaptoethanol: ME (2 mM). Deferoxamine: DFO (100 μM); Ferrostatin-1: Ferr-1(10 μM). Z-VAD-fmk: Z-VAD (10 μM). Necrostatin-1 and -2: Nec-1(5 μM) and Nec-1-2 (10 μM). Scar bar: 100 μm. d) Averaged 5637 cell numbers in different groups in a). ∗∗∗∗ p < 0.0001.

Article Snippet: Glucose oxidase (Gox), DiO, Fluorescein Phalloidin, ATP assay kit, Hoechst, EIPA, Chlorpromazine hydrochloride (CPZ), Filipin, Ferrostatin-1, Deferoxamine, Apoptosis inhibitor (Z-VAD), β-mercaptoethanol, Necrostatin-1 and -2 were purchased from MedChemExpress.

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